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. Author manuscript; available in PMC: 2023 Jan 1.
Published in final edited form as: Neuropharmacology. 2021 Nov 2;202:108860. doi: 10.1016/j.neuropharm.2021.108860

Figure 1. Targeting strategy for generation of K4A knockin mice.

Figure 1.

Oprk1 exons 3 and 4, and the floxed neomycin cassette are shown as orange and blue, boxes, respectively. The S356A /T357A /T363A /S369A (K4A) mutations are indicated by a red asterisk. Knockin was accomplished by homologous recombination and Neo cassette was self-deleted in germ cells. Genotyping is performed with F1 and R1 primers for detection of the remaining loxP site in the K4A mutant mice. The sizes of PCR products of K4A and KOR were 497 bp and 347 bp, respectively