Figure 4.

IRAK1 was required for AF-mediated IRAK1-TAK1, apoptosis, and mitochondrial fusion in Ox-LDL-induced HUVECs. 100 μM AF and IRAK1-overexpressed plasmid were applied to treat Ox-LDL-processed HUVECs, respectively. (a) p-IRAK1, IRAK1, p-TAK1, and TAK1 expressions were certified via western blotting analysis. “1” and “2” are representative of three biological repetitions. (b) Western blotting analysis of apoptosis-related proteins (Bax and caspase 3) and mitochondrial fusion-related proteins (MFN1, MFN2, and OPA1) in the treated HUVECs. “1” and “2” are representative of three biological repetitions. (c) The expression and location of p-TAK1 was determined using IF assay in the treated HUVECs. Magnification, 200×; scale bar = 20 μm.