Fig. 10.

Effects of remdesivir (RDV) on the trophectoderm (TE) and the inner cell mass (ICM) at E3.5. Embryos are treated with RDV from E2.5 to E3.5, and immunohistochemically examined for CDX2 and SOX2 proteins, which are markers for TE and ICM, respectively. A. Representative images of RDV-treated embryos that are stained for the nuclei (DAPI), CDX2 (green), and SOX2 (red). Scale bar = 50 µm. B. Numbers of total nuclei, and those of CDX2-positive and SOX2-positive nuclei. Red lines indicate mean values (n = 19 or 20 for each treatment group). Statistically significant differences are marked with horizontal bars and p-values (t-test) between two groups. C. Distribution of SOX2-positive and CDX2-positive nuclear numbers for individual embryos. Linear trend lines representing the means of the SOX2/CDX2 ratios are superimposed for each treatment group (n = 19 or 20).