Table 1.
Primer sequences for qRT-PCR analyses.
| Gene name | Primer sequences (5’ → 3’) | Characteristics | References |
|---|---|---|---|
| Amotl2 | F: TGGTTGGCTTTCCTCTGCTTTTTA R: CTGCTGGTGGGAACGAATACATTT |
TE marker (E3.5) | [37] |
| Cdx2 | F: GACTTCCTGTCCCTTCCCTCGTCT R: CCTCCCGACTTCCCTTCACCATAC |
TE marker (E3.5–4.5) | [55] |
| Eomes | F: ATCTCCTAACACTGGCTCCCACTG R: CGTTGGTCTGTGGCACGGTTCTCT |
TE marker (E4.5) | [51] |
| Esrrb | F: GTCCTTCCCTCCAGACTTGACTAC R: ACATCTTAAGTCATTCCAGCCACAAC |
EPI marker (E4.5) | [42] |
| Gapdh | F: GCATGGCCTTCCGTGTTCCT R: CCCTGTTGCTGTAGCCGTATTCAT |
Housekeeping | [37] |
| Gata3 | F: CATGCTCTGTGAATCAGTCCCTGT R: AACCCTCCAGAGTACATCCACCTT |
TE marker (E3.5–4.5) | [21] |
| Gata4 | F: AGCCAAGCCCTCTTAAGTCAGACA R: CTACAGCTCTGTGGGTGATGAGGA |
PrE marker (E4.5) | [26] |
| Nanog | F: GCTTTGGAGACAGTGAGGTGCATA R: GCTACCCTCAAACTCCTGGTCCTT |
ICM/EPI marker (E3.5/E4.5) | [41] |
| Pdgfra | F: ATGTGGTCTGCCAACCTGTACAAA R: GTTAACGTGCCTGTGGGGAATATC |
PrE marker (E4.5) | [48] |
| Pou5f1 | F: AGGCAGGAGCACGAGTGGAAAGCA R: GGAGGGCTTCGGGCACTTCAGAAA |
ICM/EPI marker (E3.5/E4.5) | [45] |
| Sox2 | F: CACATGAAGGAGCACCCGGATTAT R: CTGGAGTGGGAGGAAGAGGTAACC |
ICM/EPI marker (E3.5/E4.5) | [59] |
| Sox17 | F: ACTGCGGAGTGAACCTCTCAGACA R: GTGTGTAACACTGCTTCTGGCCCT |
PrE marker (E4.5) | [4] |
Abbreviations: EPI, epiblast; ICM, inner cell mass; PrE, primitive endoderm; TE, trophectoderm.