Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 May 9;8(5):500–512. doi: 10.1038/s41477-022-01146-6

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022, corrected publication 2022, corrected publication 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Extended Data Fig. 6 — (a) HPLC analysis of (±)-alsophilin, (−)-alsophilin, and (+)-alsophilin on a C18 OSAKA SODA CAPCELL PAK column (150 × 4.6 mm I.D., 5 µm) using water (solvent A) and acetonitrile (solvent B) as gradient eluent (0-30 min, 10%-50% B; 30-35 min, 50%-100% B), flow rate 1 ml/min, at 382 nm. (b) HPLC analysis of (±)-alsophilin, (−)-alsophilin, and (+)-alsophilin on a chiral column Daicel Chiralpak IC column (250 × 4.6 mm I.D., 5 µm) using isopropyl and hexane as eluent (60:40) at a flow rate of 1 ml/min. (c) Circular dichroism (CD) spectra of (±)-alsophilin, (−)-alsophilin, and (+)-alsophilin in MeOH, measured using JASCO J-815 CD spectro polarimeters. (d) Comparison of the calculated ECD spectra for (7′S,8′S)-alsophilin and (7′R,8′R)-alsophilin with the experimental spectra of (−)-alsophilin and (+)-alsophilin in MeOH. Energies of the conformers of (+)-alsophilin at B3LYP/6-311g (d,p) in MeOH are shown in Supplementary Table 20.