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. 2022 May 9;8(5):500–512. doi: 10.1038/s41477-022-01146-6

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© The Author(s) 2022, corrected publication 2022, corrected publication 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 1 — (a) Estimation of genome size and heterozygosity based on 23-mer frequency distribution analysis using the GenomeScope software. The genome of A. spinulosa was estimated as 6.23 Gb with the heterozygosity of 0.28%. (b) Assembly strategy of A. spinulosa genome. PacBio long reads were assembled into contigs by the Smartdenovo software. Illumina short reads were used to correct contigs. Hi-C-based scaffolding was generated using 3D-DNA pipeline, and 69 pseudo-chromosomes were obtained. The final chromosome-level assembled genome size was 6.23 Gb with scaffold N50 size of 92.48 Mb. (c) Genome-wide interaction heat map of Hi-C links among chromosome groups (69 chromosomes). Each chromosome has higher intensity of interactions with itself than any other chromosomes (Darker red color means stronger interactions).