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. 2022 May 9;8(5):500–512. doi: 10.1038/s41477-022-01146-6

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© The Author(s) 2022, corrected publication 2022, corrected publication 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 5 — (a) The first heat map shows FPKM values of 17 monolignol biosynthetic genes in pith (Pi), sclerenchymatic belt (Sb), xylem (Xy), and phloem (Ph) by RNA-seq analysis and their transcript abundance by qRT-PCR analysis. The second heat map shows FPKM values of 17 monolignol biosynthetic genes in St1 (stem stage 1), So1 (sorus stage 1), Le1 (leaf stage 1) by RNA-seq analysis and their transcript abundance by qRT-PCR analysis. Transcript abundance and FPKM values were normalized using the Z-score method. (b) Heat map shows FPKM values of five CAld5H genes in different tissues of A. spinulosa. Pi1/2/3 (pith stage 1/2/3), SPX1/2/3 (wavy structure stage 1/2/3), So1/2/3 (sorus stage 1/2/3), St1/2/3 (stem stage 1/2/3), Le1/2/3 (leaf stage 1/2/3), Ga (gametophyte). All gene accession numbers are shown in Supplementary Data 3.

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