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. 2021 Oct 16;71(6):1313–1330. doi: 10.1007/s00262-021-03076-2

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — A In SCEA database, after setting the suitable parameters (t-SNE perplexity score = 25, k value = 94), all colon cancer single cells were clustered into 94 subpopulations according to their expression patterns. B–D The key genes with specific expression patterns in single-cell level, including CA2 (B), PLAC8 (c) and TSPAN1 (D). They could act as the marker genes in cluster 9