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. Author manuscript; available in PMC: 2022 Oct 13.
Published in final edited form as: Cell Host Microbe. 2021 Sep 6;29(10):1521–1530.e10. doi: 10.1016/j.chom.2021.08.010

Figure 4. The GSDMD Pore-forming domain is the target of IpaH7.8.

Figure 4.

(A) Domain swapping strategy depicts PFD-boundaries for human and mouse GSDMD.

(B, C and E) Immunoblots of 293T cells co-transfected with IpaH7.8 (wild-type, WT, unless mutant C357A, CA is indicated) and GSDMD (wild-type, WT, or the mutants indicated). mm, mouse. hs, human. 5XA, the five residues indicated were replaced with five alanines. PFD, pore-forming domain; CTD, C-terminal domain. hs↔mm aa swap, human-mouse GSDMD chimera with indicated residues swapped in.

(D) Alignment of mouse (mm) and human (hs) GSDMD N-terminal protein sequences highlighting human residues 11–25 (red bar).

(F) Structural overlay of human (hs) GSDMD (6N9O, magenta, aa 11–25) and mouse (mm) GSDMD (6N9N, cyan, aa 11–26). N, N-terminal. C, C-terminal. mm18G is colored red.

(G) Truncation mutants of IpaH7.8(C357A) used in (H) and (I).

(H and I) Immunoblots showing co-immunoprecipitation of the IpaH7.8 LRR and GSDMD from co-transfected 293T cells. Results representative of 3 independent experiments.

See also figure S4.