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. 2022 May 20;12:8568. doi: 10.1038/s41598-022-10757-0

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Response curves of eCBome mediators and precursors (fmol/μl) to maximal aerobic test according to the diet. Panels show results for (A) N-acylethanolamines, (B) monoacylglycerols and (C) polyunsaturated fatty acids. The graph represents the mean with the standard error of the plasmatic concentrations of these lipids at each time point for the two diets. T_pre corresponds to the initial concentration before the aerobic test, T₀ corresponds to the immediate end of the aerobic test and T₁₅, T₃₀, T₄₅ and T₆₀ correspond to the recovery phase. Lipid concentrations have been normalized using ranked values fitted into mixed linear-effect models (LME) and differences between diets and times have been tested by analysis of variance (ANOVA). For difference between diets, significance was set at p < 0.1 (.), p < 0.05 (*), p < 0.01 (**) and p < 0.001 (***). For difference between times, indicated by bold blue line, significance was set at p < 0.1 (a), p < 0.05 (b), p < 0.01 (c), p < 0.001 (d). N = 7 per diet. Names of the molecules are anandamide (AEA), N-palmitoylethanolamine (PEA), N-oleoylethanolamine (OEA), Nlinoleoylethanolamine (LEA), N-stearoylethanolamine (SEA), Ndocosapentaenoylethanolamine (DPEA), Neicosapentaenoylethanolamine (EPEA), Ndocosahexaenoyl‑ethanolamine (DHEA), 1- plus 2-arachidonoyl-glycerol (2-AG), 1- plus 2-palmitoyl-glycerol (2-PG), 1- plus 2-oleoyl-glycerol (2-OG), linoleoyl-glycerol (2-LG), 1- plus 2-eicosapentaenoyl-glycerol (2-EPG), 1- plus 2-docosaepentaenoic-glycerol (2-DPG), 1- plus 2-docosahexaenoyl-glycerol (2-DHG), arachidonic acid (AA), docosahexaenoic acid (DHA), docosapentaenoic acid (DPA) and eicosapentaenoic acid (EPA).