Table 3.
Overview of the purification process.
| Protein Concentration (mg/ml) | Relative Specific Activity (% of purified DvCH3H) | Recovery (%) | Purification factor (–) | |
|---|---|---|---|---|
| Homogenization and Centrifugation | 13.6 ± 0.30 | 0.59 ± 0.08 | 73 ± 4 | – |
| Ultra Centrifugation | 5.7 ± 0.30 | 1.38 ± 0.23 | 99 ± 5 | 2.3a ± 0.3 |
| Solubilization and Ultra Centrifugation | 2.1 ± 0.10 | 1.54a ± 0.75 | 61 ± 3 | 1.1a ± 0.6 |
| IMAC Particle-based Column | 1.35 ± 0.02 | 66.7a ± 18 | 93a ± 28 | 43a ± 26 |
| SEC | 0.22 ± 0.01 | 100 | 73a ± 10 | 1.5a ± 0.4 |
| Overall | 30a ± 18 | 170a ± 23 |
aTheoretical values were calculated from indirect measurements, as indicated in the text, because direct measurements were not possible.