Table 4.

Kinetic parameters of purified recombinant membrane bound DvCH3H.

Substrates	λ (nm)	KM (µM)	vmax (µmol min−1 mg−1)	kcat (s−1)	kcat/ KM (µM−1 s−1)
(2R)-Naringenin	290	0.408 ± 0.015	0.536 ± 0.010	0.501 ± 0.01	1.228 ± 0.069
(2S)-Naringenin	290	0.76 ± 0.094	0.610 ± 0.025	0.571 ± 0.023	0.751 ± 0.124
Racemic Naringenin	290	0.402 ± 0.046	0.546 ± 0.031	0.511 ± 0.029	1.271 ± 0.219
Apigenin	346	0.329 ± 0.007	0.49 ± 0.002	0.458 ± 0.002	1.395 ± 0.033
Kaempferol	370	0.622 ± 0.069	0.457 ± 0.015	0.427 ± 0.014	0.688 ± 0.098

λ indicates the wavelength used for quantification of the reaction product.