Fig. 2. Relative abundance of Symbiodiniaceae community (ITS2) profiles.

Symbiodiniaceae community profiles in the six coral species before bleaching and 9 weeks after inoculation and the cultured Cladocopium C1^acro strain used for reinoculation. Defining intragenomic variants (DIVs) are recurring assemblages of ITS2 sequences found at set abundances across samples. Each bar represents a sample from a coral fragment (n = 3 per reinoculation treatment, n = 4 for treatment N), with each individual bar representing the proportion of a DIV sequence relative to the total abundance of sequences. Empty bars correspond to samples for which no reads were obtained or which were removed for possessing only one DIV (considered as insufficient sequence depth). No-template controls from the DNA extraction and PCR amplification (n = 3 for each) were also sequenced and are shown. Ctl− = negative control treatment where corals were chemically bleached but not reinoculated with any Symbiodiniaceae. Ctl+ = positive control treatment where corals were chemically bleached and then reinoculated with freshly isolated homologous Symbiodiniaceae. RI = reinoculation treatment where corals were reinoculated with a cultured Cladocopium C1^acro strain. RiS = reinoculation with sand treatment where corals were reinoculated as in treatment Ri in the presence of sterilised sand. N = native Symbiodiniaceae community before bleaching. * = dead corals.