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. 2022 Feb 24;16(6):1594–1604. doi: 10.1038/s41396-022-01208-9

Fig. 4. A subtractive antibiotic strategy enabled replacement of an established A. muciniphila strain.

Fig. 4

A Experimental design depicting conventionalization of germ-free mice with a negative microbiota devoid of A. muciniphila and colonization with strain BAA-835 at day −7. On day 0, mice were treated with antibiotics or regular drinking water for five days. On day 5, mice receiving antibiotics were returned to regular drinking water and fecal samples were collected. Also on day 5, mice began receiving daily gavages of YL44 for five days. Brown triangles represent timepoints for fecal sample collections. Abundance of A. muciniphila BAA-835 (dark blue) and A. muciniphila YL44 (light blue) in feces of mice not treated with antibiotics (B) or treated with CLA alone (C), D MTT alone, E AMP alone, F AMP + MTT, G AMP + MTT + CLA. Values are presented as mean ± the standard deviation. In each plot, time points with different letters are significantly (p < 0.05) different from one another by two-way ANOVA repeated measures and Tukey test multiple pairwise comparisons.