Reagent or Resource	Source	Identifier
Experimental Models: Organisms/Strains
D. melanogaster. btl-Gal4	60
D. melanogaster. btl-RFP-moe	9
D. melanogaster. UAS-ykiRNAi	Vienna Drosophila RNAi Center	VDRC: 104523
D. melanogaster. UAS-ykiRNAi	Vienna Drosophila RNAi Center	VDRC: 40497
D. melanogaster. UAS-ykiRNAi	Tsinghua Stock Center	THU0579
D. melanogaster. UAS-ykiRNAi	Bloomington Drosophila Stock Center	BDSC: 34067
D.melanogaster.UAS-yki.S168A	Bloomington Drosophila Stock Center	BDSC: 28818
D.melanogaster.UAS-CD8:GFP	Bloomington Drosophila Stock Center	BDSC: 5137
D. melanogaster. UAS-YAP-SPARK	This paper
D. melanogaster. Dl-Gal4	61
D. melanogaster. ex-lacZ	45
D. melanogaster. UAS-InR DN	Bloomington Drosophila Stock Center	BDSC: 8252
D. melanogaster. UAS-sdRNAi	Tsinghua Stock Center	THU2534
D. melanogaster. UAS-AktRNAi	Tsinghua Stock Center	THU0552
D. melanogaster. UAS-Akt	Bloomington Drosophila Stock Center	BDSC: 8191
D. melanogaster. UAS-wtsRNAi	Vienna Drosophila RNAi Center	VDRC: 106174
D. melanogaster. UAS-dlpRNAi	Bloomington Drosophila Stock Center	BDSC: 34091
D. melanogaster. UAS-bnlRNAi	Bloomington Drosophila Stock Center	BDSC: 34572
D. melanogaster. UAS-hntRNAi	62
D. melanogaster. UAS-dallyRNAi	63
D. melanogaster. UAS-NcadRNAi	Tsinghua Stock Center	THU2665
D. melanogaster. UAS-TrlRNAi	Tsinghua Stock Center	THU03912.N
D. melanogaster. UAS-AMPK DN	Bloomington Drosophila Stock Center	BDSC: 32112
D. melanogaster. yki-V5-Flag	Vienna Drosophila RNAi Center	VDRC: 318237
D. melanogaster. UAS-Flag-yki	64
D. melanogaster. UAS-ykiS168A:HA-GFP	Bloomington Drosophila Stock Center	BDSC: 28816
D. melanogaster. dally:YFP	Kyoto Stock Center	DGRC: 115511
Cell line:
HEK293T	ATCC	CRL-11268
Antibody/Kit
anti-β-galactosidase	Developmental Studies Hybridoma Bank	40-1a
anti-Hnt	Developmental Studies Hybridoma Bank	1G9
anti-Dlp	Developmental Studies Hybridoma Bank	13G8
anti-Ncad	Developmental Studies Hybridoma Bank	DN-EX
anti-Serp	65
anti-Verm	65
anti-Rho1	Developmental Studies Hybridoma Bank	P1D9
anti-MMP1	Developmental Studies Hybridoma Bank	5H7B11/3A6B4/3B8D12
anti-Thiophosphate ester	Abcam	#Ab92570
Phospho-(Ser/Thr) Kinase Substrate Antibody Sampler Kit	Cell Signaling Technology	#9920
anti-FLAG	Sigma	M2
anti-HA	ABclonal	#AE008
anti-Yki	66
anti-Bnl	67
HRP-conjugated α-mouse	Jackson ImmunoResearch	#115-035-003
HRP-conjugated α-rabbit	Jackson ImmunoResearch	#111-035-144
anti-mouse Alexa Fluor®488	Jackson ImmunoResearch	#115-545-003
anti-rabbit Alexa Fluor®488	Jackson ImmunoResearch	#111-545-003
anti-mouse Cyanine Cy™3	Jackson ImmunoResearch	#115-165-003
anti-rabbit Cyanine Cy™3	Jackson ImmunoResearch	#111-165-003
anti-mouse Alexa Fluor®647	Jackson ImmunoResearch	#115-605-003
anti-rabbit Alexa Fluor®647	Jackson ImmunoResearch	#111-605-003
ATP kit	Promega	#G7570
Glucose kit	APLLYGEN	#E1011
Click-iT® EdU Imaging Kits	Invitrogen	#C10337
SMART-Seq v4	Takara Bio
RNeasy Micro Kit	Qiagen	#74004
Software and Algorithms
ZEN (blue edition)	Carl Zeiss	N/A
ImageJ 1.53n	NIH	https://imagej.net
GraphPad Prism	GraphPad Software	https://www.graphpad.com/scientific-software/prism/
Hisat2		https://ccb.jhu.edu/software/hisat2
DESeq2	Bioconductor	https://bioconductor.org/
Homer 4.11		https://homer.ucsd.edu/homer/
pyGenomeTracks 3.5.1		https://pygenometracks.readthedocs.io/
ChIPseeker 1.22.1	Bioconductor	https://bioconductor.org/
PANTHER		http://pantherdb.org/