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. 2022 May 20;13:2833. doi: 10.1038/s41467-022-30465-7

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a Representative off-target discovery of ABR-001 and SpCas9 by TTISS at VEGFA Target 1. b–d TTISS analysis across 6 test loci for each of 3 genes. Teal (ABR-001) and blue (SpCas9) bars show average on-target indel activity for n = 2 replicates, each indicated by an outlined circle. Doughnut plots depict proportion of detected cleavage sites for each nuclease. Off-targets are shown as light gray wedges, while the on-target site is highlighted in teal (ABR-001) or blue (SpCas9). Centered numerical values represent fraction of on-target TTISS reads. Source Data are provided as a Source Data file.