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. 2022 May 20;13:2833. doi: 10.1038/s41467-022-30465-7

Fig. 4. ABR-001 mediates highly efficient genome editing when delivered via AAV vector.

Fig. 4

a Schematic of the AAV vector backbone for delivery of ABR-001 effector and a guide crRNA. b Indel rates at 72 h following transduction of ABR-001 and SaCas9 AAVs at increasing MOI in HEK293T cells. Teal (ABR-001) and gray (SaCas9) shaded bars represent the average of n = 2 bioreplicates, each indicated by an outlined circle. c Indel rates at two target sites measured daily for 7 days after transduction of HEK293T cells with ABR-001 AAVs at GC:Cell ratios of 1.6e4, 3.2e4, and 1.3e5. Shaded bars represent average indel rate of n = 2 bioreplicates for GC:Cell ratio of 3.2e4 and n = 1 bioreplicate for GC:Cell ratio of 1.6e4 and 1.3e5. Measurements for individual bioreplicates are indicated by an outlined circle. d ABR-001-AAV indel profiles across two targets and 2 MOIs over a time course of 7 days. The color scale indicates the fraction of indel reads at a given indel size. Source Data are provided as a Source Data file.