TABLE 3.

Numbers of clc, cba, and fcb isolates; their substrate ranges; and genetic properties

Genotypea	No. of isolates distributed by sampling siteb						Substrate rangec			RFLP pattern(s)d	HMW plasmide	IS1071f
	Total	BRC	DHC	Well	GW	SBR	3-CBA	4-CBA	3,4-DCBA
clcABD	15	6	0	3	4	2	12	6	0	ii–vi	8	6
cbaABC	9	6	2	1	0	0	8	4	8	i	4	9
fcbB	4	4	0	0	0	0	0	4	0	ii–iv	2	3

^aGenotypes are as described for Table 1. 

^bAbbreviations for isolate sources: BRC, isolates from samples of the sediment-water interface of Bloody Run Creek (this site was previously shown to be contaminated by Hyde Park leachate [35]); DHC, isolates from the Devil’s Hole Creek groundwater seep contaminated by Hyde Park leachate (35); Well, isolates from sampling wells drilled into the unconsolidated wastes at Hyde Park; GW, isolates from contaminated groundwater collected from the perimeter trench around the Hyde Park site; SBR, isolates from the sequencing batch reactors used for treating contaminated groundwater at Hyde Park (49). 

^cNumbers of isolates able to degrade selected CBA congeners. Substrate abbreviations are as described in the footnotes to Tables 1 and 2. Some isolates degraded more than one CBA congener. 

^dRFLP patterns grouped according to the fragment sizes following restriction enzyme digestion as follows. For clcABD, NdeI restriction fragments that hybridized with the clcABD probe were as follows: i, 19.5, 9.0, and 1.6 kb (detected in the control strain AC866 only); ii, 7.4 kb; iii, 9.5 and 5.7 kb; iv, 13.3, 10.3, and 5.7 kb; v, 10.3 and 5.7 kb; vi, 7.1 kb. For cbaABC, EcoRI restriction fragments that hybridized with the cbaABC probe represented one pattern only: i, 1.4- and 1.1-kb fragments (as for the control strain BR60). For fcbB, EcoRI restriction fragments that hybridized with the fcbB probe were as follows: i, 2.0 and 1.6 kb (detected in the control strain CBS3 only); ii, 4.9 kb; iii, 11.2 kb; and iv, 3.4 kb. 

^eNumber of isolates containing plasmids greater than 30 kb in size. HMW, high molecular weight. 

^fNumber of isolates containing the IS1071 3.1-kb NheI restriction fragment detected by hybridization with the HindIII fragment H4 probe.