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. 2022 Mar 8;16(6):1657–1667. doi: 10.1038/s41396-022-01214-x

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — Dark teal are fractions with minimum sizes of 0.22 μm or higher. Light teal are fractions with a maximum size of 0.22 μm or lower. a Barchart of the proportion of samples with at least one marine jumbo phage (x-axis) by size fraction (y-axis) sorted from highest to lowest. b Boxplot with x-axis as the number of marine jumbo phages found in a sample with size fraction on the y-axis sorted by median. c Boxplot with x-axis as the relative abundance of marine jumbo phages found in a sample (RPKM) with size fraction on the y-axis sorted by median. Significance bars in c, d correspond to Wilcox tests, with stars corresponding to p values < 0.05 (* < 0.05, ** < 0.01, *** < 0.001, **** < 0.0001) and those with p values > 0.05 as not significant "ns" (stat_compare_means function). NMDS plots (Bray Curtis dissimilarity distances) of jumbo phage composition in each sample using presence absence data (d) and relative abundance data (e). Samples are colored by size fraction distinction above 0.22 μm (dark teal) and below 0.22 μm (light teal). Ellipses calculated based on multivariate normal distribution.