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. 2022 Apr 22;115(6):821–837. doi: 10.1007/s10482-022-01735-7

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — Overview of the bacterial genera cultured from gut samples of black solder fly larvae (BSFL) by the use of two different cultivation methods, dilution-to-extinction enrichment cultivation and direct plating cultivation. A, B Genera distribution according to the detected phylotypes from all isolates cultured by direct plating (A) and dilution-to-extinction cultivation (B). Values were calculated based on a total of 138 isolates for the direct plating (A) and 341 isolates for the dilution-to-extinction cultivation (B). C Phylotype (defined based on 16S rRNA gene sequence data, Fig. 2) distribution according to the employed media (R2A, ½ R2A, TS, LB) and cultivation approaches. The numbers in C represent the numbers of studied isolates derived from individual colonies. R1-R3 represent studied replicates. Each replicate represented a pool of ten BSFL guts