TABLE 2.
Antibodies
| Antigen/Cell Type | Antibody/Clone Name | Species/Isotype | Immunostain Concentration | Source |
|---|---|---|---|---|
| ATF3 (C-19) | SC-188 | Rb P | 1:200 | Santa Cruz Biotechnology, Dallas, TX |
| ATF4 (pSer245) | NB100-81802 | Rb P IgG | 1:100 | Novus Biologicals, Littleton, CO |
| ATF6 | LS-B2516 | Rb P | 1:200/1:500 | LifeSpan Biosciences, Seattle, WA |
| β-2 microglobulin (FL-199) | SC-15366 | Rb P | 1:50 | Santa Cruz Biotechnology |
| Calnexin | ab2595 | Rb P | 1:500 | Abcam, Cambridge, MA |
| Caspase-3, active (cleaved form) | AB3623 | Rb P | 1:50 | Millipore, Burlington, MA |
| Caspase-12 | ab62484 | Rb P | 1:500 | Abcam |
| CNPase (OGC) | C9743 | Rb P | 1:150 | Sigma-Aldrich, St. Louis, MO |
| EIF2S1 (phosphor S51) [E90] | ab32157 | Rb mAb IgG | 1:100 | Abcam |
| GADD 153 (R-20) | SC-793 | Rb P | 1:400 | Santa Cruz Biotechnology |
| Iba-1 (Macrophages/Microglia) | 09-19741 | Rb P | 1:250 | Wako Diagnostics USA, Mountain View, CA |
| MBP 82-87 | MAB 386 | Ra mAb IgG2a | 1:1000 | Sigma |
| MBP 119-131 | MCA1845 | M mAb IgG1 | 1:100* | Serotec, Oxford, UK |
| MHC Class I H2Db (28–14-8) | ab25228 | M mAb IgG2aκ | 1:200* | Abcam |
| MHC Class II (M5/114.15.2) | NBP1-43312 | Ra mAb IgG2b | 1:100 | Novus Biologicals |
| NFκB p65 (A) | SC-109 | Rb P | 1:200 | Santa Cruz Biotechnology |
| PERK (H-300)† | SC-13073 | Rb P | 1:200 | Santa Cruz Biotechnology |
| XBP1 | LS-B188 | Rb P | 1:200 | LifeSpan Biosciences |
CNPase, 2′,3′-cyclic-nucleotide 3′-phosphodiesterase; mAb, monoclonal antibody; M, mouse; P, polyclonal antibody; Ra, rat; Rb, rabbit.
*
Using mouse-on-mouse kit (Vector).
†
This antibody has been discontinued by the supplier.