FIG. 1.
Restriction maps of the ThMA gene clones. (A) The box represents the 2.9-kb SacI DNA fragment cloned in pThMA119. The black box indicates the location of the ThMA structural gene, directing from left to right, and the lines represent the vector. (B) For overexpression and easy purification of ThMA, the structural gene on the NdeI*-HindIII DNA fragment was fused to six histidines in frame under the control of the BLMA promoter (PBLMA) by subcloning it into p6xHis119 at the NdeI and HindIII sites. The NdeI* site was introduced into the ThMA gene for convenience in subcloning.