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SDS-PAGE of purified ThMA. The apparent molecular mass of ThMA purified by a traditional chromatographic procedure (panel A, lane 1) and that of six-His-ThMA purified by using an Ni-NTA column (panel B, lane 2) from the E. coli lysate (panel B, lane 1) were approximately 64,000 Da. Standard size markers (lanes M) were myosin (205 kDa), β-galactosidase (116 kDa), phosphorylase b (97.4 kDa), bovine serum albumin (66 kDa), ovalbumin (45 kDa), and carbonic anhydrase (29 kDa).
