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TLC analysis of ThMA hydrolysis activity. ThMA was reacted with various substrates (0.5% [wt/vol]), including β-cyclodextrin (lane A), soluble starch (lane B), pullulan (lane C), panose (lane D), maltotriose (lane E), and acarbose (lane F). The reactions were carried out at 60°C for 12 h. Maltodextrins (MD) of G1 to G7, PTS, acarbose (Acb), and isoacarbose (IAcb) were used as standards (Std).
