Fig. 5.
Effects of TNF-α on the expression of chemerin chemokine-like receptor 1 (CMKLR1), leukotriene B4 receptor 1 (BLT1), and FPR2 in MH7A cells. MH7A cells were stimulated with 10 ng/mL TNF-α for 1, 6, 12, 24, or 48 h (A) and in the presence of 1, 10, and 50 ng/mL TNF-α for 12 h (B). The mRNA levels of CMKLR1 were determined using real-time PCR. Data are presented as the mean ± SEM of three independent experiments (*P < 0.05, **P < 0.01 vs. 0 h). MH7A cells were incubated for 24 h with 0–50 ng/mL TNF-α. The expression of CMKL1 and BLT1 was determined by western blot analysis (C, D, and E). The mRNA level of FPR2 was determined in the presence of 10 ng/ml TNF-α for 12 h using real-time PCR (F). The expression of FPR2 in the presence of 10 ng/ml TNF-α for 24 h was determined by western blot analysis (G). Data are presented as the mean ± SEM of three independent experiments (*P < 0.05 vs. control. †P < 0.05 vs. TNF-α).