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. 2022 Apr 18;298(6):101946. doi: 10.1016/j.jbc.2022.101946

Figure 2.

Figure 2

Serum and adipose tissue LIF levels are increased in obese mice and human.A, relative mRNA levels of Lif in tissues of C57BL/6J mice (n = 5). (B–F) C57BL/6J mice were fed normal chow diet (NCD, n = 6) or high-fat diet (HFD, n = 7) for 16 weeks. B, relative mRNA levels of Lif in brown adipose tissue (BAT), inguinal white adipose tissue (iWAT), and epididymal white adipose tissue (eWAT) in NCD and HFD mice. C, protein levels of LIF in iWAT determined by ELISA in NCD and HFD mice. D, protein levels of LIF in eWAT determined by ELISA in NCD and HFD mice. E, serum LIF concentration in NCD and HFD mice. F, association between serum LIF concentration and eWAT LIF levels in mice (n = 13, including 6 NCD mice and seven HFD mice). G–H, relative mRNA levels of Lif in stromal vascular fraction (SVF) and adipocyte fraction (Ad) isolated from eWAT from C57BL/6J mice were fed NCD (n = 5) or HFD for 12 weeks (n = 3). I, relative mRNA levels of LIF in subcutaneous adipose tissue (SAT) from normal subjects and obese patients (n = 6). J, relative mRNA levels of LIF in visceral adipose tissue (VAT) from normal subjects (n = 3) and obese patients (n = 4). Data are presented as mean ± SD. ∗p < 0.05; ∗∗p < 0.01, ∗∗∗ p < 0.001. For F, spearman correlation analysis was used. LIF, leukemia inhibitory factor.