Figure 2.

I-ATXP antagonist and 5-FU chemotherapy induced cell cycle arrest on G0/G1 phase in HepG2, SMMC-7721, and HKCl-C3 liver cancer cells. Cells were treated with dosages of 0.125 mg/ml, 0.25 mg/ml, 0.5 mg/ml, and 1 mg/ml of I-ATXP mock as negative control, and 0.2 mg/ml 5-Fu as positive control. Cells were then incubated at 37°C for 24 hours and were assayed by Flow Cytometry, indicating the percentage of (A) HepG2 cells, (B) SMMC-7721 cells, and (C) HKCl-C3 cells in various cell cycle phases. The results of three independent experiments are shown. Significant differences relative to untreated control are indicated as follows: *P < 0.05, **P < 0.005, ***P < 0.0005 and ****P < 0.00005.