Figure 8.

HepG2 liver cancer cells-derived exosome induced migration in HepG2 cells. 1×10^6 HepG2 cells were added into 6-well plates for 24 hours until a monolayer formed. Cells were treated with 20 µg/ml of Mitomycin for 3 hours. On the cell surface, we drew a vertical line using a 200 µl pipet and washed it with PBS three times. Cells were treated with various concentrations of HepG2 derived exosome (10 µg/ml and 20 µg/ml) at 37°C for 12 hours and were monitored to determine percent closure by microscopy. (A) HepG2 cells were treated with PBS for 0 hour and 12 hours; (B) HepG2 cells were treated with 10 µg/ml of exosomes for 0 hour and 12 hours; (C) HepG2 treated with 20 µg/ml of exosomes for 0 hour and 24 hours, and (D) cells percent closures were obtained after cells were treated with exosomes for 12 hours (11.5%, 26.34%, and 39.5%). Significant difference relative to untreated control are indicated as follows: *P < 0.01, ***P < 0.0004. The images were taken via microscopy: magnification, ×100.