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. 2022 Feb 23;13(6):1829–1842. doi: 10.1016/j.jcmgh.2022.02.011

Figure 2.

Figure 2

Acute hGly2–GLP-2 treatment induces entry of jejunal Lgr5+ ISCs into S-phase. (A) Representative images of Lgr5+ ISCs in early and late S-phase, as determined by the full and speckled nuclear distribution of EdU, respectively, using higher magnification of the images shown in Figure 1. (B) Percentage of eGFP+EdU+ ISCs in early vs late S-phase. (C) Acute pulse-chase protocol; the green mouse indicates use of Lgr5-eGFP-IRES-creERT2 animals (created using Biorender.com). (D–G) Percentage of eGFP+ ISCs (D) not in S-phase (BrdU-EdU-), (E) entering S-phase (BrdU-EdU+), (F) remaining in S-phase (BrdU+EdU+), and (G) exiting S-phase (BrdU+EdU-). (H) Representative images of the 4 different GFP+ cell types, as indicated by the white arrows: (a) BrdU-/EdU- (cells not in S-phase), (b) BrdU-/EdU+ (cells entering S-phase), (c) BrdU+/EdU+ (cells that remained in S-phase), and (d) BrdU+/EdU- (cells that exited S-phase). (B and D–G) N = 4–6. ∗P < .05, ∗∗P < .01, and ∗∗∗P < .001. DAPI, 4′,6-diamidino-2-phenylindole.