Table 1.
Primary and Secondary Antibodies Used in Immunofluorescent Staining of Formalin-Fixed and Paraffin-Embedded Jejunal Cross-Sections
| Primary antibody | Primary antibody source, product number, and RRID | Dilution | Secondary Alexa Fluor conjugated antibody | Secondary antibody source, product number, and RRID | Nikon swept field confocal laser line |
|---|---|---|---|---|---|
| Rabbit anti-GFP | Invitrogen, A11122, AB_221569 | 1:100 | Goat anti-rabbit IgG Alexa Fluor 488 | ThermoFisher Scientific, A-11008, AB_143165 | 488 nm |
| Mouse anti-pH3 | Cell Signaling (Whitby, Canada), 9706S, RRID not available | 1:400 | Goat anti-mouse IgG Alexa Fluor 555 | ThermoFisher Scientific, A-21422, AB_2535844 | 561 nm |
| Rat anti-Ki67 | Invitrogen, 14-5698-82, AB_10854564 | 1:200 | Goat anti-rat IgG Alexa Fluor 555 | ThermoFisher Scientific, A-21434, AB_2535855 | 561 nm |
| Rabbit anti-Olfm4 | Cell Signaling, 39141S, RRID not available | 1:200 | Goat anti-rabbit IgG Alexa Fluor 488 | ThermoFisher Scientific, A-11008, AB_143165 | 488 nm |
| Rat anti-BrdU | Abcam (Waltham, MA), ab6326, RRID not available | 1:200 | Goat anti-rat IgG Alexa Fluor 647 | ThermoFisher Scientific, A-21247, AB_141778 | 604 nm |
RRID, Research Resource Identifier.