(A) Experimental protocol of PA01 allergic airway inflammation.
(B) Cell frequency of eosinophils (CD45+MHCII–CD11b+CD64-Siglec-F+) and neutrophils (CD45+MHCII–CD11b+CD64-Ly6G+) out of CD45+ cells in the BAL of mice immunized with OVA +/− PA01 or LasB.
(C-E) ELISA of IL-4 (C), IL-5 (D) and IFN-γ amounts in the BAL of mice immunized as in (A).
(F-I) ELISA of total IgE (F), OVA-specific IgE (G), OVA-specific IgG1 (H) and IgG2b (I) amounts in the serum of mice immunized as in (A).
For (B-I) representative of 3 independent experiments, 3–4 mice per group. Data were analyzed by two-tailed unpaired Student’s t test. *p < 0.05; **p < 0.01; ***p < 0.001.
See also Figure S4.