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. 2022 May 20;28:58. doi: 10.1186/s10020-022-00481-w

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — Effects of Glab on high glucose (HG) induced oxidative stress and ferroptosis in vitro. The condition of DN in NRK-52E cells was induced by 30 mM glucose. A Cell apoptosis was detected by annexin V-PI double staining. B Cell viability detected by CCK-8 assay. C Lipid peroxidation was determined by the fluorescent probe C11 BODIPY 581/591. Green and blue colors indicate peroxidated lipids and nucleus respectively. The levels of (D) SOD, (E) CAT, (F) MDA, as well as (G) iron concentration were measured by commercial kits. The expression of ferroptosis markers (GPX4, SLC7A11, SLC3A2, and TFR1) at (H) mRNA and (I) protein levels were detected by RT-qPCR and western blot, respectively (^***P < 0.005, vs. the control group; ^#P < 0.05 and ^###P < 0.005, vs. the HG group)