Fig. 7.

PSGL-1-positive virions can be selectively captured by cells expressing P-selectin. A Schematic of cell capture assay: virus preparations were added to wells containing HeLa cells transiently transfected to express MAdCAM-1 or P-selectin to allow for virion capture. Post-incubation, wells were washed extensively to remove unbound virus and were assayed for the amount of captured virus using p24 detection. B Experimental results for virus preparations produced through transfection of HEK293T cells with different PSGL-1 phenotypes (0 ng, 2.5 ng, 250 ng of PSGL-1 pDNA for negative, low, and high phenotypes respectively) or through infection (C) were used at their undiluted titre. Results displayed are the mean ± SD of two independent experiments with samples tested in duplicate and are representative of three independent experiments. P values were determined using an unpaired t test with Bonferroni correction (***P < 0.001)