FIG. 3.
Nucleotide sequence of the region of pZR80 from EcoRI to the start of the functional kanamycin resistance gene aphA3. The fragment carries the promoter of the tet gene of pBR322 (Ptet) that is constitutive in Acinetobacter and part of the polylinker of pMTL21p (3) (doubly underlined). Unique restriction sites (in pZR80) that may be relevant for cloning have been marked above the polylinker. Relevant promoter elements have been marked and are shown in bold. The region that corresponds to the truncated tet′ gene of pBR322 has been underlined. The start of the aphA3 gene has been marked in italics, with the encoded initial methionine in AphA3 shown (Met).