TABLE 1.
Strain | Mutant gene(s) | xylE substi-tutionsa | XylE sub-stitutions | Phenotype |
---|---|---|---|---|
ADP1203 | None | None | None | Wild type |
ADP1204 | xylE1204 | C559T | R187C | Heat-sensitive |
ADP1205 | xylE1205 | A371G | E124G | Heat-sensitive |
ADP1206 | xylE1206 | T208C | F70L | Heat-sensitive |
xylE1306 | G853A | D285N | ||
ADP1207 | xylE1207 | T893C | L298P | Heat-sensitive |
ADP1208 | xylE1208 | A7G | K3E | Heat-sensitive |
ADP1210 | xylE1210 | T502C | F168L | Heat-sensitive |
ADP1212 | xylE1212 | T469A | Y157N | Null |
ADP1213 | xylE1213 | C413T | A138V | Heat-sensitive |
ADP1214 | xylE1214 | C12T (GCC → GCT) | Silent | Heat-sensitive |
xylE1314 | A245G | E82G | ||
ADP1215 | xylE1215 | A21G (CGA → CGG) | Silent | Leaky |
ADP1216 | xylE1216 | G168A (GTG → GTA) | Silent | Heat-sensitive |
xylE1316 | G782A | G261D | ||
ADP1217 | xylE1217 | A703G | T244A | Heat-sensitive |
ADP1219 | xylE1219 | C880T | H294Y | Heat-sensitive |
ADP1220 | xylE1220 | A185G | E62P | Null |
xylE1320 | T575C | L192P | ||
ADP1221 | xylE1221 | T502C | F168L | Null |
xylE1321 | G688A | D230N | ||
ADP1222 | xylE1222 | G39C (CTG → CTC) | Silent | Null |
xylE1322 | T542C | L181P | ||
ADP1227 | xylE1227 | T14C | V5A | Null |
xylE1327 | A730G | T244A | ||
xylE1328 | A884G | D295G |
Silent codon changes are shown in parentheses.