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. 2022 May 20;27:41. doi: 10.1186/s11658-022-00342-8

Fig. 6.

Fig. 6

The roles of RAB11B-AS1 overexpression in HCC. A RAB11B-AS1 expression in SNU-398 and HepG2 cells with RAB11B-AS1 overexpression or control was detected by RT-qPCR. B Migration ability of SNU-398 and HepG2 cells with RAB11B-AS1 overexpression or control was detected by transwell migration assay. Scale bars, 100 µm. C Invasion ability of SNU-398 and HepG2 cells with RAB11B-AS1 overexpression or control was detected by transwell invasion assay. Scale bars, 100 µm. D Cellular proliferation of SNU-398 cells with RAB11B-AS1 overexpression or control was detected by EdU assay. Scale bars, 100 µm. E Cellular proliferation of HepG2 cells with RAB11B-AS1 overexpression or control was detected by EdU assay. Scale bars, 100 µm. F Cellular proliferation of SNU-398 cells with RAB11B-AS1 overexpression or control was detected by CCK-8 assay. G Cellular proliferation of HepG2 cells with RAB11B-AS1 overexpression or control was detected by CCK-8 assay. H Cellular apoptosis of SNU-398 cells with RAB11B-AS1 overexpression or control was detected by TUNEL assay. Scale bars, 100 µm. I Cellular apoptosis of HepG2 cells with RAB11B-AS1 overexpression or control was detected by TUNEL assay. Scale bars, 100 µm. J Cellular apoptosis of SNU-398 cells with RAB11B-AS1 overexpression or control was detected by caspase-3 activity assay. K Cellular apoptosis of HepG2 cells with RAB11B-AS1 overexpression or control was detected by caspase-3 activity assay. L Weight and photograph of subcutaneous tumors formed by SNU-398 cell with RAB11B-AS1 overexpression or control. Results are shown as mean ± SD of n = 3 independent experiments (BK) or n = 6 mice in each group (L). *P < 0.05, **P < 0.01, ***P < 0.001 by Student’s t-test (AK) or Mann–Whitney test (L)