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. 2022 May 20;27:41. doi: 10.1186/s11658-022-00342-8

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — The roles of RAB11B-AS1 knockdown in HCC. A RAB11B-AS1 expression in SNU-398 and HepG2 cells with RAB11B-AS1 knockdown or control was detected by RT-qPCR. B Migration ability of SNU-398 and HepG2 cells with RAB11B-AS1 knockdown or control was detected by transwell migration assay. Scale bars, 100 µm. C Invasion ability of SNU-398 and HepG2 cells with RAB11B-AS1 knockdown or control was detected by transwell invasion assay. Scale bars, 100 µm. D Cellular proliferation of SNU-398 cells with RAB11B-AS1 knockdown or control was detected by EdU assay. Scale bars, 100 µm. E Cellular proliferation of HepG2 cells with RAB11B-AS1 knockdown or control was detected by EdU assay. Scale bars, 100 µm. F Cellular proliferation of SNU-398 cells with RAB11B-AS1 knockdown or control was detected by CCK-8 assay. G Cellular proliferation of HepG2 cells with RAB11B-AS1 knockdown or control was detected by CCK-8 assay. H Cellular apoptosis of SNU-398 cells with RAB11B-AS1 knockdown or control was detected by TUNEL assay. Scale bars, 100 µm. I Cellular apoptosis of HepG2 cells with RAB11B-AS1 knockdown or control was detected by TUNEL assay. Scale bars, 100 µm. J Cellular apoptosis of SNU-398 cells with RAB11B-AS1 knockdown or control was detected by caspase-3 activity assay. K Cellular apoptosis of HepG2 cells with RAB11B-AS1 knockdown or control was detected by caspase-3 activity assay. Results are shown as mean ± SD of n = 3 independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001 by one-way ANOVA followed by Dunnett’s multiple comparisons test