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Primers used for PCR amplification
| Primer sequence | ||
|---|---|---|
| Gene | Forward | Reverse |
| IL-6 | CTCCCAACAGACCTGTCTATAC | CCATTGCACAACTCTTTTCTCA |
| IL-1β | GCAGAGCACAAGCCTGTCTTCC | ACCTGTCTTGGCCGAGGACTAAG |
| TNF-α | ATGTCTCAGCCTCTTCTCATTC | GCTTGTCACTCGAATTTTGAGA |
| AR | TAAAGACATTTTGAACGAGGCC | GTCAGATATGGTTGAATTGCCC |
| CD29 | TACTCTGGAAAATTCTGCGAGT | ATAGCATTCACAAACACGACAC |
| INSL3 | CTAGAGCAGAGACATCTCCTG | CTGAGAAGCCTGGAGAGGAAG |
| GAPDH | ACCACCATGGAGAAGGCTGG | CTCAGTGTAGCCCAGGATGC |
