TABLE 1.
Oligonucleotides used for cloning and sequencing of nosZ gene fragments
| Oligonucleotidea | Sequenceb |
|---|---|
| Cloning primers | |
| CAMP-Nos661F | 5′CUACUACUACUACGGCUGGGGGCUGACCAA |
| CAMP-Nos1773R | 5′CAUCAUCAUCAUAURUCGAUCARCUGBUCGUU |
| Sequencing primers | |
| NosGrpA-For | 5′CGAYGCCGACTATCAGGG |
| NosGrpA-Rev | 5′CCCTGATAGTCGGCRTCG |
| NosGrpB-For | 5′GCCTGGCARGTSATVGTBGACGG |
| NosGrpB-Rev | 5′CCGTCVACBATSACYTGCCAGGC |
| NosGrpC-For | 5′CAYGAAGCRCCRYTGGTCAA |
| NosGrpC-Rev | 5′TTGACCARYGGYGCTTCRTG |
| Nos1319R | 5′GTBGGBGAVARCTTGCC |
a
All numbers are relative to the nosZ sequence of P. stutzeri Zobell.
b
B = C, G or T; R = A or G; S = C or G; V = A, C or G; Y = C or T.