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. 2022 May 18;10:e13461. doi: 10.7717/peerj.13461

Figure 1. Expression of melatonin receptors in human dermal papilla (DP) cells.

Figure 1

Human epidermal keratinocyte (HEK), human dermal fibroblast (HDF), and human dermal papilla (HDP) cells were incubated for 48 h. (A) The mRNA levels of MTNR1A and MTNR1B were assessed by quantitative reverse transcription polymerase chain reaction (qRT-PCR), and GAPDH served as an endogenous control. (B) Protein levels of MTNR1A and MTNR1B were assessed by western blotting, and β-actin served as a loading control. (C) HDP cells were incubated for 48 h in 2D and 3D culture systems, and mRNA levels of MTNR1A and MTNR1B were assessed by qRT-PCR. GAPDH served as an endogenous control. Data are presented as the mean ± standard deviation (SD) of three independent experiments, and normally distributed data were evaluated using two-way analysis of variance (ANOVA), followed by Tukey’s post hoc test; ** p < 0.01, **** p < 0.0001 versus HDP control cells (p = 0.0019, p = 0.0014, p < 0.0001, and p < 0.0001, respectively) (A), and ** p < 0.01 versus 2D cultured HDP control cells (p = 0.0052) (C).