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. 2022 May 18;10:e13461. doi: 10.7717/peerj.13461

Figure 3. Effect of melatonin receptors pathway on hair growth properties genes & protein in HDP spheroids.

Figure 3

(A–E) HDP cells were treated with 0.75 and 1 mM melatonin for 48 h. (A–D) mRNA levels of ALP, VCAN, BMP2, and WNT5A were assessed by qRT-PCR and normalized against GAPDH. (E) Protein levels of these signature genes were assessed by western blotting and β-actin served as a loading control. (F–H) HDP cells were cotreated with 1 mM melatonin with or without 50 µM luzindole for 48 h. (F and G) mRNA levels of WNT5A and BMP2 were assessed by qRT-PCR and GAPDH served as an endogenous control. (H) Protein levels of WNT5A and BMP2 were assessed by western blotting and β-actin served as a loading control. Data are presented as the mean ± SD of three independent experiments. (A–D) One-way ANOVA, followed by Turkey’s post hoc test; * p < 0.05, ** p < 0.01, *** p < 0.001 versus DMSO treated control (A, p = 0.0401; B, p = 0.0025; C, p = 0.012, and p = 0.003 respectively), and versus melatonin alone treatment control (F, p = 0.0008, p = 0.0005, and p = 0.0038; G, p = 0.0428, and p = 0.0058 respectively).