eTable 1.
Preparation Protocols for Different Molecular Weight PEGs and Polysorbate Used for SPT6
| Compound | Manufacturer and cat# | Dilution | Example for 10 mL | Details |
|---|---|---|---|---|
| PEG 300 | Sigma cat# 81162 |
No dilution | PEG is used undiluted | |
| PEG 3000 | Sigma cat# 8190151 |
50% (w/v) | 5 g compound | 5 g in 5 mL sterile water: Incubator 37°C for 2 h to dissolve. Centrifuge 500 g, 5 min. Adjust final volume to 10 mL with sterile water. |
| PEG 3350 | Lax-a-Day | 50% (w/v) | 5 g compound | 17 g diluted in 34 mL water. |
| PEG 20,000 | Sigma cat# 813300 |
.1%-20% (w/v) | 4 g compound | 4 g in 14 mL sterile water: Incubator 37°C for 2 h to dissolve. Centrifuge 500 g, 5 min. Adjust final volume to 20 mL with sterile water. 10% PEG 20,000: mix 8 mL of 20% PEG 20,000 with 8 mL sterile water 1% PEG 20,000: mix 2 mL of 10% PEG 20,000 with 18 mL sterile water 0.1% PEG 20,000: mix 2 mL of 1% PEG 20,000 with 18 mL sterile water 0.01% PEG 20,000: mix 2 mL of 0.1% PEG 20,000 with 18 mL sterile water |
| Polysorbate 80 (tween80) | Sigma cat# P1754 | 20% (v/v) | 5 mL compound | 5 mL + 20 mL sterile water. Vortex |
| Cremophor EL (PEG35) | Sigma cat#238470-1set | 50% (v/v) | 1 bottle 25 mL (25 g compound) | 25 mL + 25 mL ethanol 50% Final concentration: 527 mg/mL |
Abbreviations: PEG, polyethylene glycol; SPT, skin prick test.