Fig. 4.
Fluorescence imaging of probe BTH in HeLa cells. a) Control cells; b) Cells treated with BTH; c) Cells in group b treated with HOCl (10 μM); d) Cells pretreated with LPS (Lipopolysaccharide, an inflammatory stimulant, 1.0 μg mL−1) for 12 h and then incubated with BTH (10 μM); e) Cells pretreated with NAC (Nacetyl-L-cysteine, a hypochlorite scavenger, 500 μM) for 2 h and then treated with BTH (10 μM); f) Fluorescence intensity statistics graph of each group of cells. The time of treating cells with BTH (10 μM) and HOCl (10 μM) is both 25 min. All cells were treated at 37 °C. λex = 472 nm, λem = 485–585 nm.