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pH dependence of growth and of succinate consumption. Cells were grown in mineral salts medium supplemented with 50 mM sodium succinate at 30°C. The pH was measured continuously and kept constant at 6.3 by the addition of HCl. After 6 h (arrow 1) the pH was shifted to 7.6 by the addition of NaOH and was kept constant for 4 h before being shifted back to 6.3 (arrow 2). After 15 h (arrow 3) the pH control was switched off.
