TABLE 2.
Inhibition of [14C]succinate uptakea
Inhibitor | Inhibitor concn (mM) | Inhibition (%) |
---|---|---|
None | 0 | |
KCN | 0.1 | 40 |
1 | 70 | |
1b | >95 | |
NaN3 | 0.1 | 30 |
1 | 50 | |
1b | >95 | |
TCS | 0.01 | 95 |
0.1 | >95 | |
1 | >95 | |
Valinomycin | 0.1 | 5 |
0.05b | 15 | |
0.1b | 40 | |
Nigericin | 0.001 | 5 |
0.005 | 30 | |
0.01 | 60 | |
0.05 | 95 | |
0.1 | >95 | |
Vanadate | 0.1 | <5 |
0.1b | 40 | |
0.5b | 60 | |
1b | 80 | |
5b | >95 | |
N-Ethylmaleimide | 0.1 | 90 |
1 | >95 |
The degree of inhibition of the succinate uptake rate after 2 min of preincubation in the presence of the inhibitor was determined.
Value was obtained after 15 min of preincubation in the presence of the inhibitor.