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. 2022 May 21;8:48. doi: 10.1038/s41421-022-00395-1

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — a Western blot analysis of TM9SF1 in BGC-823 cells infected with the indicated lentivirus-based shRNAs. b, c Migration analysis of BGC-823 cells with the indicated lentivirus-based shRNAs. Representative images of invaded cells are shown (b). The invaded cells were quantified (c). Scale bars, 100 μm. d–f BGC-823 cells with the indicated lentivirus-based shRNAs were intravenously injected into SCID mice, respectively. Representative images of gross lungs and H&E-stained lung are shown (d). The lung weight (e) and the number of metastatic nodules (>1 mm) (f) were quantified. Scale bars, 1 cm. g The working model of PCIF1-dependent m⁶Am modification of TM9SF1 mRNA to promote gastric cancer progression. When PCIF1 level is high, it modifies TM9SF1 mRNA with m⁶Am leading to decreased translational efficiency of TM9SF1. The downregulated protein level of TM9SF1 enhance gastric cancer cell malignancy. And depletion of PCIF1 promotes the translation of its target mRNA TM9SF1. Ectopic TM9SF1 inhibits tumor growth and metastasis of gastric cancer cells. Data are shown as means ± SD. **P < 0.01, ***P < 0.001, Student’s t-test (c), Mann–Whitney test (e, f).