Skip to main content
. 2022 May 21;8:48. doi: 10.1038/s41421-022-00395-1
Primer names Sequences (5′-3′)
pcDNA3.1-PCIF1-FL Forward CTTGGTACCGAGCTCGGATCCATGGCCAATGAGAATCACGG
pcDNA3.1-PCIF1-FL Reverse TGCTGGATATCTGCAGAATTCTTAAGTGGGGTGAGGCTCGC
pcDNA3.1-TM9SF1-FL Forward CTTGGTACCGAGCTCGGATCCATGACAGTCGTAGGGAACCC
pcDNA3.1-TM9SF1-FL Reverse TGCTGGATATCTGCAGAATTCTCAGTCCATCTTGAGGTTAAC
pLVX-TM9SF1-FL Forward TCGAGCTCAAGCTTCGAATTCATGACAGTCGTAGGGAACCC
pLVX-TM9SF1-FL Reverse TTATCTAGAGTCGCGGGATCCTCAGTCCATCTTGAGGTTAAC
pLVX- PCIF1-FL Forward TCGAGCTCAAGCTTCGAATTCATGGCCAATGAGAATCACGG
pLVX- PCIF1-FL Reverse TTATCTAGAGTCGCGGGATCCTTAAGTGGGGTGAGGCTCGC
pLKO.1-shPCIF1#1 Forward CCGGACGACATTCCTATCAGGTTATCTCGAGATAACCTGATAGGAATGTCGTTTTTG
pLKO.1-shPCIF1#1 Reverse AATTCAAAAAACGACATTCCTATCAGGTTATCTCGAGATAACCTGATAGGAATGTCGT
pLKO.1-shPCIF1#2 Forward CCGGGGTTATCCCGAATCAAGTTCCTCTCGAGAGGAACTTGATTCGGGATAACCTTTTTG
pLKO.1-shPCIF1#2 Reverse AATTCAAAAAGGTTATCCCGAATCAAGTTCCTCTCGAGAGGAACTTGATTCGGGATAACC
pLKO.1-shTM9SF1#1 Forward CCGGCCTCGAACACTGGAAATCCATCTCGAGATGGATTTCCAGTGTTCGAGGTTTTTG
pLKO.1-shTM9SF1#1 Reverse AATTCAAAAACCTCGAACACTGGAAATCCATCTCGAGATGGATTTCCAGTGTTCGAGG
pLKO.1-shTM9SF1#2 Forward CCGGCCTGAGAAGATACGTCACAAATCTCGAGATTTGTGACGTATCTTCTCAGGTTTTTG
pLKO.1-shTM9SF1#2 Reverse AATTCAAAAACCTGAGAAGATACGTCACAAATCTCGAGATTTGTGACGTATCTTCTCAGG
EGFP-Forward TAATACGACTCACTATAAGGACTCAGATCTCGAGCTC
EGFP- Reverse TTTTTTTTTTTTTTTTTTTTTTTTCGCCTTAAGATACATTGATGAG