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. 2022 May 21;13(5):488. doi: 10.1038/s41419-022-04926-2

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — A Assessment of total and free cholesterol concentration in si-FASN (FASN siRNA#1 and #2) and si-Ctrl groups of the HeLa and CaSki cells. B Total and free cholesterol concentration in C33A-vector and C33A-FASN-OE groups. C, D Assessment of total and free cholesterol concentration in the HeLa and CaSki cells treated with different FASN inhibitors (Cerulenin, GSK, C75 and Orlistat). E Total cholesterol concentration in the HeLa and CaSki cells treated with Mock, C75, C75 + HMG-coA, C75 + AA-coA. F, G Lipid raft staining images and corresponding positive areas were analyzed in the same settings in A, B. H F-actin and phospho-FAK IF staining is shown, and p-FAK positivity and the number of filopodia were quantified in si-FASN (FASN siRNA #1 and #2) and si-Ctrl groups of HeLa and CaSki cells. I F-actin and phospho-FAK IF staining, quantification of p-FAK⁺ area and filopodia number were indicated in C33A-vector and C33A-FASN-OE groups. J, K Western immunoblot analysis of p-FAK, FAK, and β-actin after FASN knockdown (siRNA#1 and #2) and FASN overexpression (FASN-OE). *P < 0.05; **P < 0.01; ***P < 0.001.