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. 2022 May 21;13(5):488. doi: 10.1038/s41419-022-04926-2

Fig. 5. FASN regulates the c-Src/PI3K/AKT/FAK cascade via lipid rafts.

Fig. 5

A, B Signaling pathway screening by various pathway inhibitors (Rapamycin-mTOR pathway inhibitor 50 nM, Y-27632-ROCK pathway inhibitor 30 µM, LY294002-PI3K/AKT pathway inhibitor 50 µM, PP2-c-SRC pathway inhibitor 10 mM) in HeLa and CaSki cells. C, D Western blot analysis of p-c-Src, c-Src, p-Akt, and Akt expression in the si-FASN (#1 and #2) and si-Ctrl groups. E Western blot analysis of p-c-Src, c-Src, p-Akt, and Akt expression in the C33A-FASN-OE and C33A-vector groups. F, G Rescue experiments by applying a c-Src overexpression plasmid (Src OE) and MβCD. p-Fak, Fak, p-Akt and Akt expression levels were detected and analyzed. H, I Rescue experiments by applying Akt overexpression plasmid (Akt OE) and MβCD. p-Fak, Fak, p-Akt, and Akt expression levels were detected and quantified. J Co-IP experiments were conducted to analyze the interaction between FASN and c-Src with or without a lipid raft inhibitor. Protein extracts were immunoprecipitated with an anti-FASN antibody and the immunoprecipitations were assayed for c-Src. *P < 0.05; **P < 0.01; ***P < 0.001; #P < 0.05; ##P < 0.01; ###P < 0.001, MβCD + Src/Akt OE vs. MβCD + Vector.