TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Description | Reference or source |
|---|---|---|
| Strains | ||
| P. profundum | ||
| SS9 | Wild type | 10 |
| DB110 | Lac− Rifr SS9 derivative | 6 |
| EA2 | Oleic acid-auxotrophic mutant; DB110 derivative | This study |
| EA3 | Oleic acid-auxotrophic mutant; DB110 derivative | This study |
| EA10 | EPA-deficient mutant; DB110 derivative | This study |
| E. coli | ||
| DH5α | recA strain used in maintaining plasmids | 16 |
| ED8654 | Strain in which pRK2073 is maintained | 33 |
| Plasmids | ||
| pRK2073 | Helper plasmid carrying the tra genes necessary for conjugal transfer | 3 |
| pAMP1 | Plasmid containing an 885-bp internal fragment of SS9 EPA ORF3/4; Ampr | This study |
| pMUT100 | Mobilizable suicide plasmid; Kmr | 5 |
| pEA30 | 710-bp internal fragment of SS9 ORF3/4 in pMUT100; Kmr | This study |